Draw a dot plot representative of a flow cytometry test that looks at two markers. One marker is diagnostic, while the other marker is prognostic.

Identify and name your markers and their detecting antibodies.
Complete the plot as you wish and correctly interpret it. 

Cannot draw plots on these posts.

To create a dot plot representative of a flow cytometry test that looks at two markers, you will need to consider the following steps:

1. Determine the markers: In this scenario, you have mentioned that one marker is diagnostic, while the other marker is prognostic. Let's assume the diagnostic marker is CD45, a common leukocyte marker, and the prognostic marker is CD20, which is often used in lymphoma diagnosis.

2. Select the appropriate antibodies: Antibodies are used to tag specific markers for detection in flow cytometry. For CD45, you would use an antibody that specifically recognizes the CD45 antigen on leukocytes, such as anti-CD45-PE (phycoerythrin). For CD20, you would use an antibody that binds to the CD20 antigen on lymphocytes, such as anti-CD20-FITC (fluorescein isothiocyanate).

3. Prepare the dot plot: A dot plot represents the individual cells that are analyzed. Along the y-axis, you will plot the marker that is diagnostic (CD45 in this case), and along the x-axis, you will plot the prognostic marker (CD20). Each dot on the plot represents a single cell, and its position depends on the level of expression of both markers.

4. Interpret the dot plot: The dot plot will show clusters of cells that express different levels of CD45 and CD20. For example, cells that express high levels of CD45 and low levels of CD20 might be leukocytes, while cells that express high levels of CD20 and low levels of CD45 might be lymphocytes. By analyzing the pattern and distribution of cell populations, you can interpret the results of the flow cytometry test.

Please note that this is a simplified explanation, and there are many other factors and markers involved in flow cytometry analysis. Additionally, the interpretation of the dot plot will depend on the specific context and research questions being addressed in the flow cytometry experiment.