a. what are the composition in nutrient broth?
b. give 5 different types of media used for cultivation of microbes and define each?
http://www.medicotips.com/2011/07/nutrient-broth-composition-and-uses.html
http://www.google.com/#q=+media+used+for+cultivation+of+microbes
thank you very much
You are very welcome.
a. To find the composition of nutrient broth, you can follow these steps:
1. Search for the recipe of nutrient broth: Start by searching for the recipe or formulation of nutrient broth in various sources such as scientific literature, textbooks, or reliable online resources.
2. Check the ingredients: Look for the list of ingredients typically used in nutrient broth. It usually consists of water, peptone (a mixture of enzymatically digested proteins), beef extract or yeast extract, and sodium chloride (salt).
3. Determine the concentrations: Note the concentrations or amounts of each ingredient used in the nutrient broth recipe. The concentration of peptone, beef extract or yeast extract, and sodium chloride may vary depending on the specific recipe or desired use.
b. Here are five different types of media used for the cultivation of microbes along with their definitions:
1. Nutrient Agar: Nutrient agar is a solid medium containing peptone, beef extract or yeast extract, agar, and water. It provides the necessary nutrients for the growth of a wide range of microorganisms and allows their visualization as colonies on solid agar plates.
2. MacConkey Agar: MacConkey agar is a selective and differential medium mainly used for the isolation and differentiation of Gram-negative bacteria. It contains bile salts and crystal violet, which inhibit the growth of Gram-positive bacteria, and lactose and neutral red as indicators to differentiate lactose-fermenting and non-fermenting bacteria.
3. Sabouraud Dextrose Agar: Sabouraud dextrose agar (SDA) is a selective medium used for the cultivation of fungi and yeasts. It contains peptone, dextrose (glucose), agar, and may include antibiotics to inhibit the growth of bacteria and promote the growth of fungi.
4. Blood Agar: Blood agar is a differential medium commonly used for the isolation and identification of bacteria that can cause hemolysis (lysis of red blood cells). It contains agar, peptone, beef extract or yeast extract, and sheep or horse blood.
5. Lowenstein-Jensen Medium: Lowenstein-Jensen (LJ) medium is a selective and enriched medium used for the cultivation of Mycobacterium tuberculosis, the causative agent of tuberculosis. It contains malachite green, glycerol, egg, and potato starch, providing the necessary nutrients for mycobacterial growth and enabling the identification of slow-growing colonies.