How do you make a 10 ml buffer with 8M urea (mol. weight 60.06g/mol)
To make a 10 ml buffer with 8M urea, you will need to calculate the amount of urea needed based on the molarity and volume you want.
Here's how you can do it:
Step 1: Determine the moles of urea needed:
Since we know that the desired concentration is 8M, we can use the formula:
moles = concentration (M) x volume (L)
moles = 8M x 0.01 L (10 ml = 0.01 L)
moles = 0.08 moles
Step 2: Convert moles to grams:
To convert moles to grams, we need to know the molecular weight of urea, which is given as 60.06 g/mol.
grams = moles x molecular weight
grams = 0.08 moles x 60.06 g/mol
grams = 4.8048 grams (rounded to 4.8 grams)
So, you will need approximately 4.8 grams of urea to make a 10 ml buffer with 8M urea.