High-pressure liquid chromatography (HPLC) is a method used in chemistry and biochemistry to purify chemical substances. The pressures used in this procedure range from around 500 kilopascals (500,000 Pa ) to about 60,000 kPa (60,000,000 Pa ). It is often convenient to know the pressure in torr. If an HPLC procedure is running at a pressure of 3.65×108Pa , what is its running pressure in torr?
I do them this way.
3.65E8 Pa x (1 kPa/1000 Pa) = 3.65E5 kPa.
Then there are 101.325 kPa in 1 atm; convert to atm.
Finally there are 760 torr in 1 atm. Convert to torr.
To convert the pressure from pascals (Pa) to torr, you can use the following conversion factor:
1 torr = 133.3224 Pa.
To find the pressure in torr, divide the given pressure in pascals by 133.3224.
So, let's calculate:
Pressure in torr = (3.65x10^8 Pa) / (133.3224 Pa/torr)
Pressure in torr ≈ 2.7383x10^6 torr
To convert the pressure from pascals (Pa) to torr, you can use the conversion factor 1 Pa = 0.00750062 torr. Here's how you can calculate the running pressure in torr:
1. Multiply the given pressure in pascals (3.65×10^8 Pa) by the conversion factor:
3.65×10^8 Pa × 0.00750062 torr / 1 Pa = 2,737,327.3 torr
Therefore, the running pressure in torr for the HPLC procedure is approximately 2,737,327.3 torr.