how do I calculate rate velocity using
absorbence at 412/ min-1.
To calculate rate velocity using absorbance at 412 nm (nanometers) per minute, you first need to have the change in absorbance over a specific time period. The equation to calculate rate velocity is:
Rate Velocity = (Change in Absorbance) / (Time)
Here are the steps to calculate rate velocity:
Step 1: Measure the initial absorbance value (A1) at 412 nm.
Step 2: Measure the final absorbance value (A2) at 412 nm after a specific time interval.
Step 3: Calculate the change in absorbance by subtracting A1 from A2:
Change in Absorbance = A2 - A1
Step 4: Determine the time interval Δt (in minutes) over which the change in absorbance occurred.
Step 5: Divide the change in absorbance by the time interval to find the rate velocity:
Rate Velocity = (Change in Absorbance) / (Δt)
Make sure to use consistent units throughout the calculations, specifically for the absorbance values and the time interval.