To confirm that the recombinant plasmids obtained were exactly what you wanted, you need to determine the DNA sequence of the PCR products found in several of the recombinant plasmids. To do this, you use the primer 5'-CACG-3'. You carry out a set of four dideoxy sequencing reactions that include template DNA, DNA polymerase, the appropriate buffer, and the radiolabeled DNA primer. In each reaction, you include all four deoxynucleoside triphosphates (dNTPs) and a small amount of one of the four dideoxynucleoside triphosphates (ddNTPs).

The sequence of the original gene is given again below. If the recombinant plasmid is carrying this sequence, drag and drop the first 12 bands to create the pattern expected for the sequencing reactions as outlined above. Use the dotted lines as guides. The lines are numbered 1-12 for placement of the 12 bands and represent the fragments from smallest to largest.

I apologize, but as an AI text-based bot, I cannot visualize or interact with images, so I am unable to assist you in dragging and dropping bands to create a sequencing pattern. However, I can provide you with an explanation of how to carry out the procedure and interpret the results.

To determine the DNA sequence of the PCR products found in several recombinant plasmids, you can use the Sanger sequencing method, also known as dideoxy sequencing. This method uses DNA polymerase, a primer, and a mixture of deoxynucleotides (dNTPs) and dideoxynucleotides (ddNTPs) that are labeled with fluorescent or radioactive markers.

Here's a step-by-step guide on how to perform the Sanger sequencing reactions:

1. Design and synthesize a specific primer targeting the region of interest in the PCR product. In this case, the primer used is 5'-CACG-3'.

2. Prepare a set of four separate sequencing reactions, each containing the following components:
a. Template DNA: The PCR product or the cloned plasmid DNA.
b. DNA polymerase: A thermostable DNA polymerase like Taq DNA polymerase.
c. Buffer: A reaction buffer optimized for the DNA polymerase being used.
d. Radiolabeled DNA primer: The primer 5'-CACG-3' should be radiolabeled for detection purposes.
e. Deoxynucleoside triphosphates (dNTPs): All four dNTPs (dATP, dGTP, dCTP, and dTTP) are included in sufficient amounts to support DNA synthesis.

3. In each reaction, add a small amount of one of the four dideoxynucleoside triphosphates (ddNTPs), namely ddATP, ddGTP, ddCTP, and ddTTP. These ddNTPs lack a 3' OH group, thus acting as chain terminators.

4. Perform DNA sequencing reactions by subjecting the reaction mixture to cycling temperature conditions. The cycling typically consists of denaturation (94-96°C), annealing (45-65°C), and extension (60-72°C) steps. The exact temperature and time for each step depend on the DNA polymerase being used.

5. After the sequencing reactions are complete, separate the generated DNA fragments by size using a technique such as polyacrylamide gel electrophoresis (PAGE). The smaller fragments will move faster through the gel and be located closer to the bottom of the gel.

6. Visualize the separated DNA fragments using autoradiography or other detection methods appropriate for the labeled ddNTPs used.

Interpreting the results:
The DNA fragments will appear as a series of bands on the gel, with each band representing the termination point of the primer extension caused by the incorporation of a ddNTP. By comparing the position of these bands to known molecular weight standards, you can determine the sequence of the sampled DNA fragment.

To identify the first 12 bands and create the expected sequencing pattern, you would need to refer to the original gene sequence provided. Match the positions of the terminated DNA fragments (bands on the gel) with the nucleotides in the gene sequence to determine the DNA sequence of the recombinant plasmid.

If you have any specific questions or need further clarification, feel free to ask!