Post a New Question

biology, molecular

posted by .

Consider the following 45 base-pair (bp) DNA sequence:

1 10 20 30 40
| . | . | . | . | .
5’-CGCACCTGTGTTGATCACCTAGCCGATCCACGGTGGATCCAAGGC-3’
|||||||||||||||||||||||||||||||||||||||||||||
3’-GCGTGGACACAACTAGTGGATCGGCTAGGTGCCACCTAGGTTCCG-5’

Tip: If you want to see a review of PCR, we recommend this amazing animation and virtual lab made by the University of Utah.

You also have a collection of short DNA primers:

Primer 1: 5’-CGTGGA-3’
Primer 2: 5’-TGTGTT-3’
Primer 3: 5’-ATCCAA-3’
Primer 4: 5’-CCTTGG-3’
You put together several reaction mixtures, run them in the thermocycler under conditions that should produce a product if the reaction mixture is correct.

All mixtures contain:

The 45 bp DNA fragment
DNA polymerase that can function at high temperature
The necessary buffer conditions
For each mixture, indicate whether or not the PCR reaction would produce a product. If a product would be produced, give the length of that product in base-pairs (bp); if no product would be produced, give zero “0” for the length.

Reaction Added to Mixture Length of Resulting Product

dATP, dGTP, dCTP, dTTP
Primer 2
Primer 4

Respond to this Question

First Name
School Subject
Your Answer

Similar Questions

  1. Scientists PCR

    Scientists use polymerase chain reaction (PCR) to amplify a segment of DNA into many copies. to insert new nucleotide segments into a plasmid. to make a complementary strand of DNA. to link together multiple fragments of DNA WHICH …
  2. Biology

    Which of the following must occur for a mismatch error to be repaired?
  3. biology

    Consider the following 45 base-pair (bp) DNA sequence: 1 10 20 30 40 | . | . | . | . | . 5’-CGCACCTGTGTTGATCACCTAGCCGATCCACGGTGGATCCAAGGC-3’ ||||||||||||||||||||||||||||||||||||||||||||| 3’-GCGTGGACACAACTAGTGGATCGGCTAGGTGCCACCTAGGTTCCG-5’ …
  4. Biology

    PLEASE CONSIDER EACH OF THE FOLLOWING CHANGES SEPARATELY. Be sure to enter the sequence using the 3-letter abbreviations (for example, Ala-Gly-Ser), where the left most amino acid of your answer represents the first amino acid of the …
  5. BIOLOGY

    Consider the following 45 base-pair (bp) DNA sequence: 1 10 20 30 40 | . | . | . | . | . 5’-CGCACCTGTGTTGATCACCTAGCCGATCCACGGTGGATCCAAGGC-3’ ||||||||||||||||||||||||||||||||||||||||||||| 3’-GCGTGGACACAACTAGTGGATCGGCTAGGTGCCACCTAGGTTCCG-5’ …
  6. biology

    ou sequence many of the recombinant plasmids generated. You find three mutant versions of the inserted gene. (This is likely due to errors introduced during the PCR amplification.) The sequences of the gene found in the three mutants …
  7. Biology

    Consider the following 45 base-pair (bp) DNA sequence: 1 10 20 30 40 | . | . | . | . | . 5’-CGCACCTGTGTTGATCACCTAGCCGATCCACGGTGGATCCAAGGC-3’ ||||||||||||||||||||||||||||||||||||||||||||| 3’-GCGTGGACACAACTAGTGGATCGGCTAGGTGCCACCTAGGTTCCG-5’ …
  8. Biology

    5’-CGCACCTGTGTTGATCACCTAGCCGATCCACGGTGGATCCAAGGC-3’ ||||||||||||||||||||||||||||||||||||||||||||| 3’-GCGTGGACACAACTAGTGGATCGGCTAGGTGCCACCTAGGTTCCG-5’ Tip: If you want to see a review of PCR, we recommend this amazing animation …
  9. Biology

    5’-CGCACCTGTGTTGATCACCTAGCCGATCCACGGTGGATCCAAGGC-3’ ||||||||||||||||||||||||||||||||||||||||||||| 3’-GCGTGGACACAACTAGTGGATCGGCTAGGTGCCACCTAGGTTCCG-5’ Tip: If you want to see a review of PCR, we recommend this amazing animation …
  10. molecular biology

    To confirm that the recombinant plasmids obtained were exactly what you wanted, you need to determine the DNA sequence of the PCR products found in several of the recombinant plasmids. To do this, you use the primer 5'-CACG-3'. You …

More Similar Questions

Post a New Question