If you use the lmax during the calibration plot (Beer's plot) and then change to some other wavelength for the measurement of the sample, how will this affect x?
x is the unknown mols of H20
The lmax value in the calibration plot (Beer's plot) refers to the wavelength at which the absorbance is maximum for a particular species. This value is obtained by measuring the absorbance at various wavelengths and finding the maximum absorbance.
When you change the wavelength for the measurement of the sample, it will affect the x value, which is the concentration of the analyte being measured. This is because the absorbance of a species at different wavelengths can vary, and the Beer-Lambert law, which relates absorbance to concentration, relies on a specific wavelength at which the molar absorptivity (ε) is constant.
If you measure the sample at a wavelength different from the lmax used during calibration, the absorbance may differ, potentially leading to an inaccurate determination of concentration. This is because the molar absorptivity is wavelength-dependent, and using a different wavelength might cause deviations from the linear relationship between absorbance and concentration.
To minimize this impact, it is crucial to use the same wavelength for sample measurement as used during calibration (preferably the lmax), ensuring accurate determination of concentration using the calibration plot.