The following chemicals have the indicated formula weights:Sucrose-342.3,Potassium dihydrogen phosphate(KH2PO40-136.09,Tris -121.14.You also have the following solutions made:20%SDS,5M NaCl,500mM EDTA.You have a 40%glycerol solution and 10X TBE.List the steps to make the following buffers.a)1 liter of a solution containing :0.5Msucrose,0.05M NaCl,0.02 M KH2PO4
To make a 1-liter solution containing 0.5M sucrose, 0.05M NaCl, and 0.02M KH2PO4, you will need the following steps:
1. Calculate the mass of each chemical needed:
- Sucrose: (0.5M) x (342.3 g/mol) = 171.15 g
- NaCl: (0.05M) x (58.44 g/mol) = 2.922 g
- KH2PO4: (0.02M) x (136.09 g/mol) = 2.722 g
2. Prepare a sufficient volume of deionized water to make a 1-liter solution.
3. Measure out the calculated mass of sucrose (171.15 g) and add it to the deionized water.
4. Measure out the calculated mass of NaCl (2.922 g) and add it to the solution.
5. Measure out the calculated mass of KH2PO4 (2.722 g) and add it to the solution.
6. Stir the solution until all the chemicals are dissolved.
7. Adjust the final volume to 1 liter by adding more deionized water if needed.
8. The resulting solution will contain 0.5M sucrose, 0.05M NaCl, and 0.02M KH2PO4.
Remember to wear proper protective equipment and follow safe laboratory practices when handling chemicals.
To make a 1 liter solution containing 0.5 M sucrose, 0.05 M NaCl, and 0.02 M KH2PO4, follow these steps:
1. Calculate the required mass of each chemical:
- Sucrose: 0.5 M * formula weight of sucrose
- NaCl: 0.05 M * formula weight of NaCl
- KH2PO4: 0.02 M * formula weight of KH2PO4
2. Convert the mass to the required volume of each chemical:
- Use the formula weight to convert the mass to moles
- Then divide the moles by the molarity to get the required volume in liters
3. Determine the final volume of the solution:
- Add the volumes calculated in step 2 for each chemical together
- Ensure the total volume is 1 liter
4. Prepare the solution:
- Dissolve the calculated mass of each chemical in distilled water, adding them one by one.
- Once all chemicals have dissolved, bring the final volume up to 1 liter using distilled water.
- Mix the solution well to ensure even distribution of the components.
Note: It is important to accurately weigh and measure each chemical and to use distilled water for preparation. Additionally, safety precautions should be followed while handling chemicals.
For specific calculations, you will need to refer to the formula weights provided and perform the calculations accordingly.