If the calibration curve for a Beer's Law plot of a protein assay had a slope of 0.433 when absorb acne, A, was plotted as a function of protein concentration, c, in mg/mL. The absorbance of an unknown protein sample was 0.345. What is the concentration of protein in this sample in mg/mL?

Should say absorbancy *, A, was plotted..

My phone autocorrected the word.

A = abc; slope = 0.433 which is a.

Then 0.345 = 0.433*c. solve for c in mg/mL.

Thank you!

I solved for c and received c = 0.797
Would this answer be correct?

To find the concentration of protein in the unknown sample, we can use Beer's Law equation, which states that the absorbance (A) is directly proportional to the concentration of the solute (c). The equation can be written as:

A = ε * b * c

where:
A = absorbance
ε = molar absorptivity or the slope of the calibration curve
b = path length of the cuvette (constant)
c = concentration of the solute (protein) in mg/mL

In this case, the slope of the calibration curve (ε) is given as 0.433 and the absorbance (A) of the unknown sample is 0.345.

We can rearrange the Beer's Law equation to solve for the concentration (c):

c = A / (ε * b)

Now, substituting the values:

c = 0.345 / (0.433 * b)

The path length of the cuvette (b) is not mentioned in the question, so we cannot calculate the exact concentration without that information. However, if you have the value of the path length, you can substitute it into the equation to find the concentration.