The production of a polypeptide involves the correct sequencing of amino acids, originally encoded by a segment of DNA. This code is transcribed onto mRNA and subsequently translated using tRNA.
From the following DNA segment, record the base sequence that would be encoded on both mRNA and tRNA.
mRNA:
tRNA:
To determine the base sequence encoded on both mRNA and tRNA, we first need to convert the DNA segment into its complementary RNA sequence.
The DNA bases are adenine (A), cytosine (C), guanine (G), and thymine (T). In RNA, thymine is replaced by uracil (U).
Let's say the DNA segment is:
5'- T A A G C C A T G A T C G T A -3'
To obtain the mRNA sequence, we simply replace each DNA base with its complementary RNA base. Thus, the mRNA sequence would be:
3'- A U U C G G U A C U A G C A U -5'
For the tRNA sequence, we need to find the anticodon, which is the complementary sequence to the mRNA codon. In tRNA, the base pairing rules are slightly different. Adenine (A) pairs with uracil (U), cytosine (C) pairs with guanine (G), guanine (G) pairs with cytosine (C), and uracil (U) pairs with adenine (A).
Considering the mRNA sequence we obtained earlier:
3'- A U U C G G U A C U A G C A U -5'
The tRNA sequence would be:
5'- U A A G C C A U G A U C G U A -3'
So, the base sequence encoded on both mRNA and tRNA would be:
mRNA: A U U C G G U A C U A G C A U
tRNA: U A A G C C A U G A U C G U A