I'm doing a lab for gel electrophoresis and we added the digests in the wells of the agarose gel
Lane 2 – λ Hind III (a standard)
Lane 4 – Tube 1
Lane 5 – Tube 2
Lane 6 – Tube 3
Lane 7 – Tube 4
Now will DNA in tube 1 which only has dh20, 10x buffer, and no enzyme show on the agarose gel. In tube 2, HindIII and in tube 3, BamHI and lastly in tube 4, EcoRI are cut by restriction enzymes. I think only lane 6 as well as lane 2 and 4 show on the agarose gel. But somehow I'm confused with the results I obtain. Can DNA, and EcoRI show on the agarose gel?
To determine which DNA fragments will show on the agarose gel, we need to consider the components of each tube and the properties of the restriction enzymes used.
In tube 1, there is no enzyme present to cut the DNA. Therefore, the DNA in this tube will not be cleaved into fragments and will not show on the gel.
In tube 2, HindIII is present. HindIII is a restriction enzyme that recognizes a specific DNA sequence and cuts the DNA at that site. When HindIII is added to the DNA in tube 2, it will cleave the DNA into fragments. These fragments should show on the agarose gel.
In tube 3, BamHI is present. Like HindIII, BamHI is a restriction enzyme that recognizes a specific DNA sequence and cuts the DNA at that site. When BamHI is added to the DNA in tube 3, it will cleave the DNA into fragments. These fragments should also show on the agarose gel.
In tube 4, EcoRI is present. EcoRI is another restriction enzyme that recognizes a specific DNA sequence and cuts the DNA at that site. When EcoRI is added to the DNA in tube 4, it will cleave the DNA into fragments. These fragments should also show on the agarose gel.
Therefore, the expected results on the agarose gel would be:
Lane 2 – λ Hind III (a standard)
Lane 4 – DNA fragments from Tube 2 (cleaved by HindIII)
Lane 5 – DNA fragments from Tube 3 (cleaved by BamHI)
Lane 6 – DNA fragments from Tube 4 (cleaved by EcoRI)
It is important to note that the size and number of fragments will depend on the specific DNA sequence being cut by the restriction enzyme. If the DNA sequence does not contain recognition sites for HindIII, BamHI, or EcoRI, then no fragments will be generated in the respective tubes, and they will not show on the gel.