This is the homework exercise:
You want to add 0.13 ul of DNA to 4.87 ul of extraction mix to have a final total of 5.0 ul of sample. But you don't want to make 0.13 aliquots because it is too small. Make a dilution to accomodate for the small aliquot.
Any help would be great thanks!
To accommodate the small aliquot of 0.13 ul, you can create a dilution by adding a larger volume of a diluent to your DNA sample. Here's how you can calculate the dilution:
Step 1: Calculate the dilution factor
To determine the dilution factor, divide the final volume by the initial volume. In this case, you want a final volume of 5.0 ul and your initial volume is 0.13 ul.
Dilution factor = Final volume / Initial volume
Dilution factor = 5.0 ul / 0.13 ul = 38.46
Step 2: Calculate the dilution volume
To calculate the volume of the dilution needed, subtract the initial volume from the final volume.
Dilution volume = Final volume - Initial volume
Dilution volume = 5.0 ul - 0.13 ul = 4.87 ul
Therefore, you need to add 4.87 ul of a diluent (such as water or buffer) to your 0.13 ul of DNA to obtain a final volume of 5.0 ul. This will allow for a larger volume that is more easily handled and pipetted accurately.