Chemistry 3A

posted by .

You want to make the following buffer: 5% glucose, 100 mM tris and 10 mM EDTA in a final voume of 300ml. Your stock solutions are 20% glucose, 1M tris and 0.5M EDTA. The diluent is distilled water. Determine how much of each you will add to make the desired buffer.

I know this so far::

1- Glucose
DF = C2 / C1 = 5% (DIVIDE) 20% = 0.25

V1 - DF X V2 = 0.25 X 300 ml = 75 ml


2 - Tris Stock = 1 M = 1000 mM

DF = C2 / C1 = 100 mM (DIVIDE) 1000 mM = 0.1

V1 - DF X V2 = 0.1 X 300 ml = 30 ml

Is this right so far?

  • Chemistry 3A -

    Yes, both are right. Good work.

  • Chemistry 3A -

    I am doing the same problem.
    I got as far as you, including:

    DF = C2/C1 = 10 mM /500mM = 0.02 EDTA
    V1= DF x V2 = 0.02 x 300mL = 6 mL

    ...Now i am not sure what to do with this information. I would appreciate the help.

  • Chemistry 3A -

    add up the three volumes:
    75mL + 30mL + 6 mL = 111mL
    The question tells us that the total final volume of the buffer is 300mL, so add enough water to your solute to bring it up to that desired volume:

    300mL - 111mL = 189mL

    So you would combine 111mL of the solutes to 189mL of the diluent, which is water, to get a final volume of 300mL.

Respond to this Question

First Name
School Subject
Your Answer

Similar Questions

  1. chemistry

    An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced. What is the ratio of TRIS …
  2. Biochem

    Calculate the amount of Tris(hydroxlmethyl)aminomethane(Tris) necessary to make 100mL of a 0.100 M Tris buffer that is equimolar in Tris-base (MW=121.1 g/mol) and Tris-HCL (MW= 157.6g/mol)
  3. Biochemistry

    How do you prepare 100 mL of a 0.1 M TRIS-HCl buffer with a pH 7.4 using solid TRIS base (MW 121.1) and 1 M hydrochloric acid?
  4. Science

    You wish to make 1L of sterile lysis buffer soln. that is 10mM Tris-EDTA (pH 8) and 2% SDS. You have plenty of the following stock solns.: 2M Tris-EDTA (pH 8) and 20% SDS. However, SDS cannot be autoclaved and must be added to the …
  5. Chemistry

    Starting with solid Tris base (FW 121.1) and 1 M HCl, describe how you would make 2 liters of 200 mM Tris-HCl buffer pH 7.5. pKa of Tris 8.14
  6. chemistry

    How much Tris-Acetate-EDTA (TAE) stock buffer (3.25 M) is required to make 400 ml of solution that has a concentrations of 250 mM?
  7. Biology

    You have a stock solution with following components: glucose: 44 g/L NaCl: 191 g/L Tris Buffer: 275 g/L You mix 71 mL of the solution with water and bring the resulting solution to a final volume of 750 mL. What is the final concentration …
  8. chemistry

    . An enzyme-catalyzed reaction was carried out in a 0.2M �Tris� buffer, pH 7.8. As a result of the reaction, 0.03 mole/liter of H+ was produced. (pKa of Tris =8.1) (a) What was the ratio of Tris+ (conjugate acid) / Triso …
  9. biochemistry

    how many grams each of tris and tris HCl are needed to make up 500ml of 1m tris buffer with a PH of 8.0. pka of tris =8.3 mwt of tris =121 mwt of tris HCl =175
  10. Biochemistry

    You are asked to make 50 mM Tris-HCl pH 7.5. The pK of Tris is 8.1. What is the concentration of the components (Tris and Tris-H+) in the final buffer solution?

More Similar Questions