The highest point on a trail, a pass with elevation of 1116 m, can be attained by climbing a 3.20 mile route from a waterfall, elevation of 60 m.

The elevation gain from the waterfall to the pass is ___ feet.

Do I just subtract 1116 - 60 = 1056 m and convert to feet? What is the significance of the 3.20 mile route?

Yes.

There is no significance to the length of the route unless you wish to calculate average slope, which is not asked for.