starting with 1000 copies of a template, 35 cycles of PCR were performed. What is the theoretical yield of copies of double stranded product of this amplification reaction?
To calculate the theoretical yield of copies of the double-stranded product of the PCR amplification reaction, you need to understand how PCR works and how amplification is achieved in each cycle.
Here's how you can calculate the theoretical yield in this situation:
1. In a PCR amplification reaction, each cycle doubles the number of template copies. Therefore, for each cycle, the number of template copies is multiplied by 2.
2. In this case, you start with 1000 copies of the template. So, after the first cycle, the number of template copies becomes 1000 x 2 = 2000.
3. After the second cycle, the number of template copies becomes 2000 x 2 = 4000.
4. This doubling process continues for a total of 35 cycles. So, after the 35th cycle, the number of template copies can be calculated as 1000 x (2^35).
To calculate this:
- You can use a calculator or a spreadsheet program to calculate 2^35, which equals 34,359,738,368.
- Finally, you multiply this number by the initial number of template copies (1000) to find the theoretical yield of the double-stranded product after 35 cycles:
34,359,738,368 x 1000 = 34,359,738,368,000.
Therefore, the theoretical yield of copies of the double-stranded product after 35 cycles of PCR amplification in this case is 34,359,738,368,000 copies.