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Total plasma volume is important in determining the required plasma component in blood replacement therapy for a person undergoing surgery. Plasma volume is influenced by the overall health and physical activity of an individual. Suppose that a random sample of 48 male firefighters are tested and that they have a plasma volume sample mean of x = 37.5 ml/kg (milliliters plasma per kilogram body weight). Assume that σ = 8.00 ml/kg for the distribution of blood plasma.

Find the sample size necessary for a 99% confidence level with maximal error of estimate E = 2.40 for the mean plasma volume in male firefighters. (Round up to the nearest whole number.)
male firefighters

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