Post a New Question

chemistry

posted by .

Prepare 150 ml of a reaction buffer containing 0.75% MgCl2, 35mM glucose, 750 nM ATP, and 20 mM Tris-Cl, pH 7.5. In addition, you have a 20% MgCl2 stock solution and the lab freezer has 10 mM ATP that you can dilute to the correct concentration.

  • chemistry -

    The MgCl2 you get by c1v1 = c2v2 where c is concn and v is volume.
    glucose you get by mols = grams/molar mass.
    ATP is c1v1 = c2v2
    tris-cl you get by use the Henderson-Hasselbalch equation in a manner similar to your last post.

Respond to this Question

First Name
School Subject
Your Answer

Similar Questions

  1. chemistry lab

    An enzyme-catalyzed reaction is carried out in a 50-mL solution containing 0.1 M TRIS buffer. The pH of the reaction mixture at the start was 8.0. As a result of the reaction, 0.002 mol of H+ were produced. What is the ratio of TRIS …
  2. Chemistry/hydrolysis of ATP

    Cells use the hydrolysis of ATP as a source of energy. The conversion of ATP to ADP has a standard free energy change of -30.5 kJ/mol. If all the free energy from the metabolism of glucose. C6H12O6(s)+6O2(g)¨6CO2(g)+6H2O(l) goes …
  3. muscle physiology

    okay so we did a lab on muscle physiology. acquired one small 2 cm length of psoas muscle . teased the bundle of muscle fibers into individual muscle fibers. aligned 3 individual fibers on each of three different microscope slides …
  4. biochemistry

    1. Walking consumes approximately 100 kcal/mi. In the hydrolysis of ATP (ATP → ADP + Pi), the reaction that drives the muscle contraction, ΔGo’ is -7.3 kcal/mol (-30.5 kJ/mol). Calculate how many grams of ATP must be produced …
  5. biochem

    You have the following stock solutions and solid chemicals in your lab: 1.5M NaCL 0.2M Tris buffer, pH 8.0 10 mg/ml Bovine serum albumin (BSA) MgCl2 (solid, anhydrous) MW = 95.211 g/mole (95% pure) How would you make 250 ml of the …
  6. chem biochem bio

    You have the following stock solutions and solid chemicals in your lab: 1.5M NaCL 0.2M Tris buffer, pH 8.0 10 mg/ml Bovine serum albumin (BSA) MgCl2 (solid, anhydrous) MW = 95.211 g/mole (95% pure) How would you make 250 ml of the …
  7. Molecular Bio

    You are planning to digest human genomic DNA with the EcoRI restriction enzyme but realize you do not have any 10X buffer available to add to the reaction. The components of the 10X buffer are: 50 mM NaCl, 100 mM Tris, 10 mM MgCl2, …
  8. Chemistry

    In order to make 500.0mL of a 1.835 M MgCl2 solution, then _______g of MgCl2 is needed. Moles of MgCl2= (1.835M)(0.5L)=0.9175mol Molecular Weight of MgCl2= 95.211 g/mol (0.9175mol MgCl2)(95.211 g/mol)= 87.36g of MgCl2 Did I do this …
  9. Biology

    You have a stock solution with following components: glucose: 44 g/L NaCl: 191 g/L Tris Buffer: 275 g/L You mix 71 mL of the solution with water and bring the resulting solution to a final volume of 750 mL. What is the final concentration …
  10. Chemistry

    Cells use the hydrolysis of adenosine triphosphate (aTP) as a source of energy. This reaction can be written as, ATP(aq) + H2O(l) --> ADP(aq) + H2PO4-(aq), wehre ADP represents adenosine diphosphate. For this reaction, Delta Grxn …

More Similar Questions

Post a New Question