Chemistry

posted by .

I conducted a Gas Chromatography experiment using Flame Ionisation Detector (FID).A capillary column was used.
1)Blank
2) Standard
3) Sample
4)Blank
5)Standard
The above was injected into the system.

In 2)Standard chromatogram,a sharp, thin peak was observed between the 2 principal peaks. This peak had area count of less than 100.
This sharp peak was also seen in 4)Blank.

However, this sharp peak was not present in 5)Standard chromatogram.
Can you suggest a reason for this sharp spike/peak seen in 2) and 4)?

  • Chemistry -

    I would suspect contamination.

  • Chemistry -

    Dear Bob,
    Can the sample results be accepted then if we exclude the "integration" of this "peak" from the chromatogram?

  • Chemistry -

    I'm not sure what kind of quantitative work you are doing, I would think it could be accepted if the outlying peak was not integrated with the other two peaks AND if the total of the three are not used somewhere.

Respond to this Question

First Name
School Subject
Your Answer

Similar Questions

  1. statistics

    A maintenance supervisor is comparing the standard version of an instructional booklet with one that has been claimed to be superior. An experiment is conducted in which 26 technicians are divided into two groups, provided with one …
  2. Chemistry - please help

    A substance was tested for Impurity A using liquid chromatography. 2 determinations were performed. In the 1st chromatogram, Impurity A was not detected, i.e. no Impurity A peak was observed and in the 2nd chromatogram, Impurity A …
  3. Chemistry-help

    Hi, I have a question on Gas Chromatography(GC). The following were injected into the GC system: - 1) 1% sample solution in acetone 2) a 500 times dilution of item 1) The retention time of the main peak in the diluted sample solution …
  4. Chemistry

    What is the function of Compressed Air in GC-FID (i.e. Gas Chromatography-Flame Ionization Detector)?
  5. Chemistry

    I have 2 questions on columns used in the Gas Chromatography-Flame Ionisation Detector (GC-FID) instrument: - 1) If a capillary column is left in the GC-FID instrument for 2 months with the instrument switched off (i.e. instrument …
  6. Chemistry

    In a gas chromatography experiment, an internal standard was used. Which is considered the blank in this experiment:- 1) Internal standard in diluent or 2) Diluent only or are 1) & 2) both acceptable?
  7. chemistry

    The percent water in methyl alcohol can be determined by gas chromatography using a capillary column coated with a non-polar stationary phase and a thermal conductivity detector. A series of calibration standards yielded the following …
  8. chemistry

    You have purifiedLDH using affinity chromatography. Using the bradford assay you determined the total protein concentration of the column fraction to be 10 mg/ml in a final volume of 2.4 ml. You diluted some of your column fraction …
  9. Organic Chemistry

    Gas Chromatography: The internal standard technique is used. A volatile component which does not interfere with the GC analysis (i.e. it gives a separate peak) is added to the sample which is then analyzed. For example, 10.0 mg of …
  10. Chemistry

    What is the mobile phase in this experiment?

More Similar Questions