When looking at a sample of blood for White Blood Cell's, what could you do to reduce interference from Red Blood Cells?
What happens when you look through a blue filter?
rbcs are the smaller in size than compared to wbc so we can filter them with ultra filters like millipore filters
To reduce interference from red blood cells when looking at a sample of blood for white blood cells, you can follow these steps:
1. Dilution: Take a small amount of blood and dilute it with an appropriate diluent. This will help to disperse the red blood cells, thereby reducing their concentration in the sample.
2. Red blood cell lysis: Use a red blood cell lysis solution or buffer to break down the red blood cells. This will release the hemoglobin and other components of the red blood cells, allowing you to separate the white blood cells more easily.
3. Density gradient separation: Utilize a density gradient separation technique, such as centrifugation using a density gradient medium. This method separates the different types of blood cells based on their density. By carefully layering the blood sample on top of the density medium and centrifuging it, the white blood cells will move towards a specific layer, while the red blood cells settle elsewhere.
4. Immunomagnetic separation: Apply immunomagnetic separation using specific antibodies directed against red blood cells. This technique uses magnetically labeled antibodies that bind specifically to the red blood cells, allowing for their removal while leaving the white blood cells intact.
By employing these techniques, you can minimize the interference caused by red blood cells and accurately analyze the white blood cells in the blood sample.