Compound A is provided as a 500mM solution. If 12.5 mL is taken and added to 12.5mL of water, how many moles (or millimoles / micromoles) are there per 0.1mL of the diluted solution?
To calculate the number of moles (or millimoles / micromoles) per 0.1 mL of the diluted solution, we need to calculate the final concentration of Compound A in the diluted solution.
First, we need to determine the amount of Compound A that was added to the diluted solution:
12.5 mL of Compound A solution was taken.
Next, we need to determine the amount of Compound A in moles:
We know that the Compound A solution concentration is 500 mM, which means 500 millimoles of Compound A are present in 1 liter (1000 mL) of solution.
Therefore, the number of moles in 12.5 mL of Compound A solution is:
(12.5 mL / 1000 mL) × (500 mM / 1) = 0.00625 moles
Now, we can calculate the final volume of the diluted solution:
12.5 mL of Compound A solution + 12.5 mL of water = 25 mL
To determine the concentration of Compound A in the diluted solution, we divide the number of moles by the final volume:
0.00625 moles / 25 mL = 0.00025 moles/mL
Finally, we can calculate the number of moles (or millimoles / micromoles) per 0.1 mL of the diluted solution:
(0.00025 moles/mL) × (0.1 mL / 1) = 0.000025 moles (or 0.025 millimoles / 25 micromoles) per 0.1 mL of the diluted solution.
To find out the number of moles (or millimoles/micromoles) per 0.1 mL of the diluted solution, we need to first calculate the final concentration of Compound A after dilution.
Let's break down the steps:
1. Calculate the amount of Compound A in the diluted solution:
Since 12.5 mL of Compound A is mixed with 12.5 mL of water, the total volume of the solution is 12.5 mL + 12.5 mL = 25 mL.
2. Calculate the final concentration of the diluted solution:
Divide the amount of Compound A by the total volume of the solution.
Concentration of Compound A = (Amount of Compound A) / (Total volume of solution)
We know that the concentration of Compound A in the provided solution is 500 mM (mM = millimolar).
Concentration of Compound A = 500 mM = 500 mmol/L
To obtain the concentration in moles/mL, divide the concentration in mM by 1000.
Concentration of Compound A = 500 mmol/L ÷ 1000 = 0.5 mol/L
To calculate the concentration in mol/mL (millimoles/mL), divide the concentration in mol/L by 1000.
Concentration of Compound A = 0.5 mol/L ÷ 1000 = 0.0005 mol/mL
3. Determine the number of moles (or millimoles/micromoles) per 0.1 mL of the diluted solution:
Multiply the concentration of Compound A by the volume (0.1 mL) to obtain the number of moles (or millimoles/micromoles) of Compound A in 0.1 mL.
Number of moles (millimoles/micromoles) of Compound A per 0.1 mL = (Concentration of Compound A) × (Volume of diluted solution)
Now, substitute the values into the equation:
Number of moles (millimoles/micromoles) of Compound A per 0.1 mL = 0.0005 mol/mL × 0.1 mL
Calculate the result:
Number of moles of Compound A per 0.1 mL = 0.00005 moles
Therefore, there are 0.00005 moles (or 0.05 millimoles or 50 micromoles) of Compound A per 0.1 mL of the diluted solution.