3. Compound A is provided as a 500mM solution. If 12.5 mL is taken and added to 12.5mL of water, how many moles (or millimoles / micromoles) are there per 0.1mL of the diluted solution?
moles = M x L
moles = 0.250 x 0.0001 L = ?
That anonymous post was mine.
It's 500 mM to start. You dilute it 12.5 to 25 which makes it now 250 mM or 0.250 moles/L. So how many moles are in 0.1 mL?
I don't know what is it
please show me how to do it DrBob222
Thank you
To calculate the number of moles (or millimoles/micromoles) per 0.1 mL of the diluted solution, we need to follow a few steps:
Step 1: Determine the number of moles in the initial 12.5 mL of Compound A solution.
To do this, we can use the formula:
Number of moles = (Concentration in moles per liter) x (Volume in liters)
Given that the compound is provided as a 500 mM solution and we have 12.5 mL, we need to convert the volume to liters:
Volume in liters = 12.5 mL / 1000 mL/ L = 0.0125 L
Plugging this information into the formula, we get:
Number of moles = (500 mM) x (0.0125 L) = 6.25 millimoles
Step 2: Calculate the total volume of the diluted solution.
Since we are adding 12.5 mL of Compound A to 12.5 mL of water, the total volume of the diluted solution is 12.5 mL + 12.5 mL = 25 mL.
Step 3: Determine the volume of the diluted solution we are interested in (0.1 mL).
We know that we have a total volume of 25 mL, and we want to find the number of moles (or millimoles/micromoles) per 0.1 mL of the diluted solution. So, we need to calculate the conversion factor:
Conversion factor = (Number of moles in the diluted solution) / (Volume of diluted solution)
Number of moles in the diluted solution = Number of moles in the initial solution (6.25 millimoles)
Plugging in the numbers, we get:
Conversion factor = (6.25 millimoles) / (25 mL) = 0.25 millimoles per mL
Since we want to find the number of moles (or millimoles/micromoles) per 0.1 mL, we multiply the conversion factor by 0.1:
Number of millimoles per 0.1 mL = Conversion factor x 0.1 = 0.25 millimoles per mL x 0.1 mL = 0.025 millimoles
Therefore, there are 0.025 millimoles (or micromoles) per 0.1 mL of the diluted solution.