Chemistry-Bob please help
posted by anne .
Posted by anne on Friday, October 14, 2011 at 6:30pm.
Hi, I have a question on HPLC (high performance liquid chromatography).
The procedure says to use a
4.6 x 150mm x 3um column.
The relative retention time of Impurity B with reference to the main peak is about 0.5 (i.e the ratio of the retention times is 0.5)
If I use a 4.6 x 100mm x 2.7um column, will the relative retention time of Impurity B with reference to the main peak still be about 0.5?
My calculations below, show yes, about the same, but it has been a long time since I did that. I have asked DrBob to take a look at it to, I am uncertain if he has done any HPLC work.
I haven't done any HPLC work.