I did a lab with catalase breaking down hydrogen peroxide. A question asks, what gas is being formed (oxygen), suggest a method I could use to determine this.
I don't know any method?
Stick a burning wood splinter into the gas. It blazes rapidly (more cncentrated O2)
To determine the gas being formed during the catalase reaction, you can use a simple method called the "inverted gas jar method." Here's how you can perform this experiment:
Materials needed:
1. Catalase solution
2. Hydrogen peroxide (H2O2)
3. Gas jar with a narrow neck
4. Delivery tube
5. Bowl or basin filled with water
6. Candle or splint
Procedure:
1. Set up a water bath by filling a bowl or basin with water.
2. Fill the gas jar with water, completely immersing it in the water bath to remove any air bubbles.
3. Place the delivery tube into the gas jar, ensuring that the open end of the tube is below the water level.
4. Prepare a catalase solution by mixing it with hydrogen peroxide.
5. Carefully pour the catalase solution into the gas jar while keeping the delivery tube submerged.
6. Observe the reaction taking place in the gas jar.
7. After a few moments, you will see gas bubbles being formed.
8. Ignite a candle or a splint (Use caution and follow safety precautions).
9. Gently remove the delivery tube from the gas jar, keeping the open end underwater.
10. Bring the open end of the delivery tube close to the flame.
11. If the gas is oxygen, you will observe a glowing splint or a candle flame relighting when placed near the open end of the tube.
12. Record your observations and conclusions.
By using the inverted gas jar method, you can collect the gas formed during the catalase reaction and test it to determine if it is oxygen. If the flame relights when brought close to the gas, it confirms the presence of oxygen.