1. You are given three fat samples and told that one of them contains cholesterol. State and clearly outline the test you would use to identify which of these samples contains cholesterol. Hint: Name of test, Procedure of test, and Results.

To identify which of the three fat samples contains cholesterol, you can perform a colorimetric test called the Liebermann-Burchard test. Here is a clear outline of the test procedure and the expected results:

Test: Liebermann-Burchard Test for Cholesterol
Procedure:
1. Prepare three test tubes labeled A, B, and C.
2. Take a small amount of each fat sample and dissolve it in a suitable solvent like chloroform or ethyl acetate.
3. Transfer each fat solution into the corresponding test tube.
4. Add a few drops of acetic anhydride to each test tube. Caution: Acetic anhydride is corrosive, so use proper safety precautions.
5. Heat the test tubes in a water bath or hot plate at around 50-60 degrees Celsius for about 1-2 minutes.
6. Take out the test tubes and allow them to cool to room temperature.
7. Add a few drops of concentrated sulfuric acid (H2SO4) to each test tube, drop by drop, while gently shaking the tubes.
8. Observe the color changes in each test tube.

Expected Results:
- Sample A (Without Cholesterol): The solution in test tube A will remain colorless or turn slightly yellow due to the reaction of acetic anhydride and H2SO4.
- Sample B (With Cholesterol): The solution in test tube B will turn green, and the intensity of the color indicates the amount of cholesterol present.
- Sample C (Without Cholesterol): The solution in test tube C will remain colorless or turn slightly yellow, similar to sample A.

Interpretation:
The development of a green color in test tube B indicates the presence of cholesterol in the respective fat sample. Test tubes A and C serve as control samples to verify the absence of cholesterol.

Please note that this explanation is for educational purposes, and if you want to conduct such tests, ensure you have proper knowledge, training, and safety measures in place.