How to prepare a 0.8% agarose gel with TAE. It does not give the concentration of the TAE so I am not sure how to prepare this gel.
Thanks
To prepare a 0.8% agarose gel with TAE (Tris-Acetate-EDTA) buffer, you need to know the concentration of the TAE buffer. However, if the concentration of TAE is not provided, you can assume a typical concentration of 1X, which is commonly used for DNA electrophoresis.
Here are the steps to prepare a 0.8% agarose gel with 1X TAE:
1. Determine the volume of the gel you want to prepare. Let's assume you want to prepare 100 mL of gel.
2. Calculate the total amount of agarose needed. For a 0.8% agarose gel, you need 0.8 grams of agarose per 100 mL of gel. So, for 100 mL of gel, you would need 0.8 grams of agarose.
3. Add the calculated amount of agarose to a flask or beaker.
4. Add the desired volume of 1X TAE buffer to the flask. Typically, a 1X TAE solution is prepared by diluting a concentrated TAE stock solution. If you don't have a concentrated TAE stock solution, you can prepare it by mixing Tris base, acetic acid, and EDTA.
5. Gently swirl or stir the mixture to ensure the agarose is evenly dispersed.
6. Place the flask in a microwave or heat source to dissolve the agarose completely. Heat the mixture in short intervals (around 20 seconds) and stir in between to prevent boiling over.
7. Once the agarose is fully dissolved, let the mixture cool down until it is warm but not hot to touch. If it is too hot, it may solidify too quickly.
8. Pour the warm agarose mixture into a gel casting tray. Make sure to remove any air bubbles by gently tapping the tray.
9. Insert the desired comb or well-forming tool into the gel, creating wells for loading samples.
10. Allow the gel to solidify completely, which may take around 20-30 minutes at room temperature.
Now, your 0.8% agarose gel with TAE is ready for DNA electrophoresis.