I don't know where to start with it?
A tube is set up for radioimmunoassay containing antibody with a capacity for binding 1ug antigen. Both labelled (50,000 counts per ug) and unlabelled antigen are added to the tube. Work out a formula to calculate the amounts of counts bound to antibody in each case.
Any help will be greatly appreciated!
To calculate the amounts of counts bound to the antibody in each case, you can use the following formula:
Counts Bound = Amount of Antigen × Counts per ug
In this formula, "Amount of Antigen" refers to the quantity of antigen present, and "Counts per ug" refers to the number of counts per microgram of the labeled antigen.
Let's break down the equation further to understand it better.
1. Amount of Antigen:
You mentioned that the tube has a capacity for binding 1 ug (microgram) of antigen. Therefore, the "Amount of Antigen" will be the quantity of antigen added to the tube in micrograms.
2. Counts per ug:
You mentioned that the labeled antigen has 50,000 counts per ug. This means that for every microgram of labeled antigen, there are 50,000 counts associated with it.
Putting it all together, we can now use the formula:
Counts Bound = Amount of Antigen × Counts per ug
For example, let's say you added 0.5 ug of labeled antigen to the tube. Using the formula, the calculation would be:
Counts Bound = 0.5 ug × 50,000 counts per ug
Counts Bound = 25,000 counts
So, in this case, there would be 25,000 counts bound to the antibody.
Similarly, you can use this formula to calculate the counts bound for unlabelled antigen by replacing the "Counts per ug" value with the appropriate count value for the unlabelled antigen.
I hope this helps! Let me know if there's anything else I can assist you with.