posted by Holly .
We cut a slice of the target gene from the gel which ran electrophoresis. And then purified the gene from the gel by using Gel-M column, WN buffer and WS buffer. After these steps, we ran another electrophoresis of the purified gene and there was no band showed on the picture. What might happened to make this situation happened?
This is not my area but hopefully some of the following sites will help you: