what amount of ammonium chloride do I need to make a 100 micromolar solution?
To determine the amount of ammonium chloride needed to make a 100 micromolar solution, you will need to know the molar mass of ammonium chloride (NH4Cl) and the desired final volume of the solution.
Here's a step-by-step process to calculate the amount:
1. Determine the molar mass of ammonium chloride (NH4Cl). The molar mass can be calculated by summing the atomic masses of each element in the compound.
Atomic Mass of N = 14.01 g/mol
Atomic Mass of H = 1.01 g/mol
Atomic Mass of Cl = 35.45 g/mol
Molar Mass of NH4Cl = (4 x Atomic Mass of H) + Atomic Mass of N + Atomic Mass of Cl
2. Convert the desired concentration from micromolar (µM) to moles per liter (M). To convert, divide the micromolar concentration by 1,000,000.
Conversion: 100 µM = 100/1,000,000 M
3. Calculate the number of moles of ammonium chloride needed. Multiply the concentration in moles per liter (M) by the desired final volume of the solution in liters.
Number of Moles = Concentration (in M) x Volume (in L)
4. Finally, calculate the mass of ammonium chloride needed using the molar mass and number of moles.
Mass = Number of Moles x Molar Mass
Note: It's important to ensure that the volume used in the calculation is consistent with the desired final volume mentioned earlier.
By following these steps, you can calculate the amount of ammonium chloride required to make a 100 micromolar solution.