I had a Bio lab about gel electrophoresis and PCR. for each lab, we have to make up thoughtful, detailed questions. You don't have to answer the questions so please don't go through the trouble by answering them.
The first question is about something we did in lab. My question is What soy based food product is a GMO or a non-GMO product?
Second question is a follow up question and the question has to be beyond the scope and something you can test for outside of class. My question is How would you know if there's another GMO product present if the bands are the same size?
The third question is that is associated with the topic in general. I'm kind of having trouble coming up with a question for that. For example, for my photosynthesis lab, my question was : Light energy is needed for photosynthsis to occur; what amount of light is needed to initiate the reaction?
Do you have any questions that you can come with? It can be about PCR or gel electrophoresis. It doesn't matter. If you don't mind, you don't have to write a topic question. Just write whatever comes to your mind and maybe I can use to trigger something in my brain.
Sure! Here are a couple of questions related to PCR and gel electrophoresis that you can consider:
1. How does the number of PCR cycles impact the amount of target DNA amplified? What factors can influence the efficiency of PCR amplification?
To answer this question, you can perform a PCR experiment using different cycle numbers and then analyze the resulting PCR products using gel electrophoresis to compare the amount of amplification in each case. Additionally, you can manipulate various parameters like primer concentration, template DNA concentration, and annealing temperature to observe their effect on PCR efficiency.
2. Is it possible to determine the sequence of a DNA fragment by using only gel electrophoresis?
To explore this question, you can design a hypothetical DNA fragment with known sequence, perform PCR to amplify it, analyze the PCR product on a gel, and then use gel electrophoresis data to infer the sequence of the band(s). You can compare your inferred sequence with the actual sequence to evaluate the accuracy of gel electrophoresis in sequence determination.
These questions should prompt further thinking and experimentation in the field of PCR and gel electrophoresis. Good luck with your lab project!