The reason you might see fewer colonies on your LB/Amp/Arabinose + plate (compared to the number of colonies..?
The reason you might see fewer colonies on your LB/Amp/Arabinose + plate (compared to the number of colonies that you see on your LB/Amp + plate) is because?
a. UV light slows the growth of the colonies
b. Expression of a large protein like GFP could slow transcription of other essential cellular proteins
c. Arabinose turns off expression of beta-lactamase
d. A and B
e B and C
Not positive on this. Please help ! thanks!
i want to know about the funtion of the cell lables and what they do
To determine the reason why you might see fewer colonies on your LB/Amp/Arabinose + plate compared to the number of colonies on your LB/Amp + plate, let's break down the options provided:
a. UV light slows the growth of the colonies: UV light is not mentioned as a factor in the question, so this option can be ruled out.
b. Expression of a large protein like GFP could slow transcription of other essential cellular proteins: This option suggests that the production of a large protein like GFP might have an impact on the transcription of other essential cellular proteins. If this is the case, it could potentially lead to slower growth or decreased viability of the bacterial colonies, resulting in fewer colonies on the LB/Amp/Arabinose + plate.
c. Arabinose turns off expression of beta-lactamase: This suggests that arabinose, a sugar molecule, could inhibit the expression of beta-lactamase. Beta-lactamase is an enzyme that confers resistance to ampicillin, which is contained in the LB/Amp medium. If the expression of beta-lactamase is turned off by arabinose, it would render the ampicillin ineffective in selecting for bacteria containing the antibiotic resistance gene. This could result in fewer colonies on the LB/Amp/Arabinose + plate compared to the LB/Amp plate.
Considering the explanations above, the likely reason for seeing fewer colonies on the LB/Amp/Arabinose + plate compared to the LB/Amp + plate would be option e. B and C – the expression of a large protein like GFP could slow transcription of other essential cellular proteins, and arabinose turns off the expression of beta-lactamase.