Hi - I have some data from a biology experiment that I need to find out if it is significant and I don't know what test to use - maybe Anova - but I am not sure which variables are which.

I used 4 levels of light (from dark to bright). 30 sandflies. At 4 different times I measured the distance the sand flies buried themselves into the sand and I have 4 depths I measured: 0-5.5cm, 5.6-11cm, 11.1-16.5cm and 16.6-22cm.

Can anyone advise please?
Thank you :)

What is your null hypothesis? This defines your independent and dependent variables. You want to find out how your dependent variable(s) change with variations in your independent variable(s).

Possibly this review might help:

An independent variable is the potential stimulus or cause, usually directly manipulated by the experimenter, so it could also be called a manipulative variable.

A dependent variable is the response or measure of results.

Extraneous variables — other than the independent variable — potentially can affect the dependent variable, so they must be controlled. If possible, you try to keep them constant between the experimental and control group.

Without knowing your hypothesis, this is all I can do. I hope this helps. Thanks for asking.