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chem please help

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how would you make a 4% percent paraformaldehyde solution?

starting from 20% (diluted with PBS)
so add
496 ml of PBS (accounting for 4% paraformaldehyde) or would you add 500ml of PBS

Thank you

  • chem please help -

    It depends on whether the percent is w/v or v/v.

    if it is v/v, as I suspect, you are diluting the original by a factor of five, so add 4 parts water.

    if you want 500 ml, add 100ml of 20 percent, and add 400ml water.

    if you want 45ml, add 1 part of PBS (9ml) and four parts (36ml) water.

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