I need help with designing a lab about enzymes. I want to change the amount of enxyme inhibitors as the independent variable, but I don't know what enzyme I could use or what inhibitor I could use. What are some common, easy-to-use enzymes with inhibitors?
Since this is not my area of expertise, I searched Google under the key words "enzyme inhibitors" to get these possible sources:
http://en.wikipedia.org/wiki/Enzyme_inhibitor
http://en.wikipedia.org/wiki/Enzyme
http://www.elmhurst.edu/~chm/vchembook/573inhibit.html
http://stezlab1.unl.edu/reu1999/dputn226/ChemHelp/RET_Web_Pages/Enzyme_inh/enz_ihb2.htm
Under "enzyme" I found:
http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/E/Enzymes.html
http://people.ku.edu/~jbrown/enzyme.html
http://web.indstate.edu/thcme/mwking/enzyme-kinetics.html
In the future, you can find the information you desire more quickly, if you use appropriate key words to do your own search.
I hope this helps. Thanks for asking.
When designing a lab about enzymes, it is important to consider the overall objective and the availability of resources. Enzymes are proteins that catalyze specific biochemical reactions, and there are various commonly studied enzymes with inhibitors that you can use for your experiment. Here are a few commonly used enzymes with inhibitors that could be suitable for your lab:
1. Catalase: This enzyme is involved in the breakdown of hydrogen peroxide into water and oxygen. It is widely available in various tissues, such as liver or potato tissues. Inhibition of catalase can be achieved by using substances like sodium azide or cyanide.
2. Amylase: This enzyme breaks down starch into sugars. You can obtain amylase from sources like saliva, potatoes, or malt extract. Amylase inhibitors, such as acarbose or glycerol monostearate, can be used to inhibit the action of amylase.
3. Protease: This enzyme is involved in the breakdown of proteins into amino acids. Papain, extracted from papaya, is a commonly used protease. Protease inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) or ethylenediaminetetraacetic acid (EDTA), can be employed to inhibit protease activity.
When choosing an enzyme and inhibitor, it is important to consider factors such as accessibility, safety, and the specific aims of your experiment. You may also need to consider the availability of detection methods or assays to measure enzyme activity or substrate/product concentrations.