I' am currently trying to wrie a couple of lab conclusions and having a hard time coming up with good points to make for the following lab experiment:
Thin layer chorotography
If anyone has a few minutes to give me a couple tips on how to improve this. I think it sounds like a summary with not enough anyalis of the data and what it means.
THANKS!
Here it is:
A chromatography lab experiment is used to separate chemical compounds from one another. Using flourene, fluorenol, fluorenone, two unknowns, and a reference mixture
we found how far they were able to travel from measuring their solvent fronts after development of the TLC plates. Using this information Rf vaules were calculated for flourene, fluorenol, and fluorenone, from these specific values their polarities could then be determined. Fluorene’s Rf value was 0.94 making it the least polar, fluorenol’s Rf vaule was 0.28 making it the most polar, and fluorenone’s Rf value was 0.64 making it intermediate in terms of polarity. The unknowns were discovered by comparison of the TLC plates of flourene, flurenol, and fluorenone. In the second part of the chromatography experiment there were to be timed intervals when adding the compounds to the TLC plates, this was to watch the progress and reduction of flurenone to fluorenol.
I think it sounds like a summary, also. Did you mean you thought you needed two conclusions to report in this experiment OR you have another report to write and you will need a conclusion for that one, too. See below for some coments.
A chromatography lab experiment is used to separate chemical compounds from one another. Using flourene, fluorenol, fluorenone, two unknowns, and a reference mixture
we found how far they were able to travel from measuring their solvent fronts after development of the TLC plates. Using this information Rf vaules were calculated for flourene, fluorenol, and fluorenone, from these specific values their polarities could then be determined. Fluorene’s Rf value was 0.94 making it the least polar, fluorenol’s Rf vaule was 0.28 making it the most polar, and fluorenone’s Rf value was 0.64 making it intermediate in terms of polarity. The unknowns were discovered by comparison of the TLC plates of flourene, flurenol, and fluorenone. In the second part of the chromatography experiment there were to be timed intervals when adding the compounds to the TLC plates, this was to watch the progress and reduction of flurenone to fluorenol.
Conclusions:
Thin layer chromatography (TLC) may be used to separate similar compounds. The substrate used in this experiment was aluminum oxide and the moving phase was hexane. The Rf values for fluorene, fluorenol, and fluorenone were measured; the polarity of each of the compounds was determined from the Rf value. Table ?? shows the results for Rf values as well as the polarity of each compound tested.
TLC may be used, also, to identify compounds. In this experiment, two unknowns were subjected to the same procedure described above and the Rf values were used to identify the components successfully.
ETC.
another conclusion is that you were able to watch the progress of the reduction of flurenone to fluorenol.
Don't use my wording but this will give you an idea of what I would turn in. It lists two conclusions and I didn't write about everything that was done. The table to which I refer above may have been done and it may in the procedure/results somewhere. If so, you need not print the table again; simply refer to it in the conclusions. I hope this is of some value. Perhaps other tutors will have other suggestions.
what question?
Here is an improved version of the conclusions for your chromatography lab experiment:
1. Thin layer chromatography (TLC) is an effective method for separating and identifying chemical compounds. By measuring the distance traveled by different compounds from their solvent fronts on TLC plates and calculating their Rf values, we were able to determine their relative polarities. Based on our results, fluorene was found to be the least polar compound with an Rf value of 0.94, fluorenol was the most polar with an Rf value of 0.28, and fluorenone was intermediate in polarity with an Rf value of 0.64.
2. Furthermore, TLC can be used to identify unknown compounds. In our experiment, we compared the TLC plates of the unknown compounds with the TLC plates of fluorene, fluorenol, and fluorenone. By analyzing their Rf values, we were able to successfully identify the components of the unknown compounds.
Additionally, our experiment included a second part where we observed the progress and reduction of fluorenone to fluorenol by adding compounds to the TLC plates at timed intervals.
By including these two main conclusions, you address both the separation and identification capabilities of TLC, while also mentioning the specific observation made in the second part of the experiment.
Here are a few tips to improve the conclusion of your lab experiment:
1. Provide a concise summary of the experiment: Begin the conclusion by briefly summarizing the purpose and procedure of the experiment. This will provide context for your observations and findings.
2. Analyze the results: Instead of simply listing the Rf values and polarities of the compounds, analyze what these values mean in terms of the relative polarities of the compounds. Discuss the trends and patterns observed, and explain how these results align with the known properties of the compounds.
3. Discuss the identification of unknown compounds: If you were able to successfully identify the unknown compounds based on the TLC plates and Rf values, discuss the importance and implications of this identification. Highlight the significance of using TLC as a tool for compound identification.
4. Describe any observations or findings: If there were any interesting or unexpected observations during the experiment, discuss them in the conclusion. For example, if you were able to observe the progress and reduction of fluorenone to fluorenol, explain the importance of this finding and how it contributes to the understanding of the reaction.
5. Discuss potential sources of error: Acknowledge any limitations or sources of error in your experiment. This could include variations in the development of TLC plates, inaccuracies in the measurement of solvent fronts, or inconsistencies in the calculation of Rf values. Discuss how these uncertainties may have impacted your results and suggest ways to improve the experiment in future studies.
By incorporating these points into your conclusion, you will be able to provide a more comprehensive analysis of the data and its implications.