I'm not sure what order these are when it comes to g-protein activation:
-conformational change in receptor
-hormone binds receptor
-Gs protein has GTP bound
-Alpha and gamma/beta subunits dissociate
-GTP is hydrolyzed to GDP on alpha sububit
-alpha and beta/gamma subunits reassociate
-adenylyl cyclase is activated
I believe the order is:
hormone binds receptor
conformational change in receptor
Alpha and gamma/beta dissociate
G protein bound to GTP
Adenylate cyclase is activated
GTP hyrdrolyzed
alpha and bet/gamma reassociate
im trying to do my project on biology in cells but i cant find it
To determine the correct order of events in G-protein activation, you can break down the process step by step and analyze the sequence of events based on their order. Here is the correct order of events in G-protein activation:
1. Hormone binds receptor: The signaling molecule (hormone) binds to the specific receptor on the cell membrane.
2. Conformational change in receptor: The binding of the hormone induces a conformational change in the receptor, activating it.
3. Alpha and gamma/beta subunits dissociate: The activated receptor interacts with the G-protein, causing the alpha subunit to dissociate from the gamma/beta subunits.
4. G-protein bound to GTP: Once dissociated, the alpha subunit binds to guanosine triphosphate (GTP), replacing the GDP (guanosine diphosphate) that was bound to it.
5. Adenylyl cyclase is activated: The alpha subunit, now bound to GTP, interacts with and activates adenylyl cyclase, an enzyme located in the cell membrane.
6. GTP hydrolyzed to GDP on alpha subunit: The GTP on the alpha subunit is then hydrolyzed to GDP, releasing a phosphate group. This helps in switching off the signal.
7. Alpha and beta/gamma subunits reassociate: After GTP hydrolysis, the alpha subunit reassociates with the beta/gamma subunits, forming an inactive G-protein complex.
It is important to note that G-protein activation can vary depending on the specific type of G-protein and pathway involved. It is always recommended to refer to reliable sources and scientific literature for accurate and detailed information. If you are looking for relevant sources for your biology project, consider checking research articles, textbooks, or reputable educational websites like PubMed, Khan Academy, or Nature Education. These sources can provide you with in-depth information on cellular processes such as G-protein activation.