The pKa of HEPES is 7.55 at 20 degrees C, and its MW is 238.31. Calculate the amounts of HEPES in grams and of 1.0 M NaOH in milliliters that would be needed to make 300 mL of 0.2 M HEPES buffer at pH 7.2.
September 30, 2009
drbob22 I think the reason you are getting a negative number instead of the positive you need to find the log is because you put your A and B in the places. Try inverting them. I'm sure you will get a positive. I followed your instructions to a Tee and never came up with a...
February 4, 2008