How to prepare 10% sucrose solution enriched with 0.1% phenylalanine? And also how to make the stock solution of phenylalanine for the above process.
I have prepared 10% sucrose solution by adding 10g sucrose in 100ml water , but how do i make 0.1% phenylanine in 10% sucrose solution ? Should i add 0.1g of phenylalanine in 10g sugar and mix both together with 100ml water? Please help!
If it's 10% w/v sucrose and 0.1%w/v phenylalanine w/v, you're close.
10 g sucrose and 0.1 g phenylalanine in enough water to make 100 mL solution.
To prepare a 10% sucrose solution enriched with 0.1% phenylalanine, you will need to follow these steps:
1. Start by preparing the stock solution of phenylalanine. A stock solution is a concentrated solution that can be diluted to obtain the desired concentration. In this case, you want to prepare a stock solution of phenylalanine that is 1% (w/v), so you need 1g of phenylalanine in 100ml of water.
Dissolve 1g of phenylalanine in 100ml of distilled water. Stir the solution gently until the phenylalanine is completely dissolved.
2. Once you have the stock solution, you can prepare the 10% sucrose solution enriched with 0.1% phenylalanine.
Start by adding 10g of sucrose to a container. Then, add 1ml of the stock solution of phenylalanine to the container. Mix the sucrose and phenylalanine together until the sucrose is fully dissolved.
3. Finally, add distilled water to the container to bring the total volume up to 100ml. Stir gently to ensure even distribution of the components.
By following these steps, you will have prepared a 10% sucrose solution enriched with 0.1% phenylalanine.