who prepaire tris buffer 0.2 mmol/lit
calculation
To calculate the preparation of a Tris buffer at a concentration of 0.2 mmol/L, you'll need to know the molar mass of Tris and the desired final volume of the buffer solution.
Step 1: Determine the molar mass of Tris
Tris, also known as Tris(hydroxymethyl)aminomethane, has a molar mass of approximately 121.14 g/mol.
Step 2: Calculate the moles of Tris required
To calculate the number of moles needed to prepare the buffer, divide the desired concentration (0.2 mmol/L) by 1000 to convert it to mol/L:
0.2 mmol/L ÷ 1000 = 0.0002 mol/L
Step 3: Convert moles to grams
Multiply the number of moles by the molar mass of Tris to convert to grams:
0.0002 mol/L x 121.14 g/mol = 0.0242 grams/L
Step 4: Determine the required volume
Finally, divide the mass of Tris by the desired concentration to find the required volume of Tris buffer solution:
0.0242 grams/L ÷ 0.2 mol/L = 0.121 L or 121 mL
Therefore, to prepare a Tris buffer at a concentration of 0.2 mmol/L, you will need to dissolve 0.0242 grams of Tris in a total volume of 121 mL.