Question: Describe the procedure you would follow to determine the concentration of vitamin C in a sample if the volume of DCPIP required to reach the end point were more than the 10ml graduated cylinder could hold. The titration must still be carried out in the the 10ml graduated cylinder. Be sure to include any calculations that you would need to perform.

You need to identify DCPIP and write the equation. It would help if you wrote the procedure you followed.

To determine the concentration of vitamin C in a sample when the volume of DCPIP required exceeds the capacity of a 10ml graduated cylinder, you can use a dilution technique along with some calculations. Here is the procedure you can follow:

1. Measure a known volume, for example, 10ml, of the sample containing the unknown concentration of vitamin C and transfer it into a clean container (e.g. an Erlenmeyer flask).

2. Using a pipette, transfer a smaller known volume, let's say 5ml, of the sample solution from the flask into a 10ml graduated cylinder. Record the volume of the sample used.

3. Fill the remaining part of the graduated cylinder up to its total capacity (10ml) with a suitable solvent (e.g. distilled water). Make sure to mix it well to ensure homogeneity.

4. Now, perform the titration by adding the DCPIP solution drop by drop into the graduated cylinder that contains the diluted sample solution. Observe the endpoint, where the color of the solution changes, indicating that all the vitamin C has reacted with the DCPIP.

5. Record the volume of the DCPIP solution used for the titration.

6. Since the volume of DCPIP required to reach the endpoint exceeded the capacity of the graduated cylinder, you will need to calculate the total volume of the diluted sample solution. Subtract the volume of the DCPIP solution used from the total volume of the graduated cylinder (10ml) to find the remaining volume. This will give you the total volume of the diluted sample solution.

7. To find the concentration of vitamin C in the diluted sample solution, you can set up a proportion using the volume of the diluted sample solution and the amount of vitamin C in that volume.

For example, if you started with 10ml of sample and diluted it to 10x (1:10) with water, and then used 5ml of the diluted sample for titration, you can set up the proportion:

(10ml) : (10x concentration) = (5ml) : (x concentration),

where x is the concentration of vitamin C in the diluted sample solution.

8. Solve the proportion for x by cross-multiplication and plug in the known values. This will give you the concentration of vitamin C in the diluted sample solution.

9. To find the concentration of vitamin C in the original sample, multiply the concentration of vitamin C in the diluted sample solution by the dilution factor (10 in this case) since the original sample was diluted 10-fold.

Note: It is crucial to ensure that proper safety measures are followed during the entire procedure, including handling chemicals and glassware appropriately.