Once the adsorbent is packed in a liquid chromatography column, it is important that the level of the elution solvent not drop below the top of the adsorbent. Why?
I answered both of these below. If you don't like those answers you need to be more specific about what you want in the answer.
oh no man. i just want different answers to see if the answers are right. And if people agree.
I just don't want to take chances. Do you have a background in chemistry or something? I have no background in chemistry so i don't know if i'm seeing a right answer or not.
Can you elaborate more? like Don't overload the column because ...
Thanks for all your help=]
The level of the elution solvent should not drop below the top of the adsorbent in a liquid chromatography column because it ensures that the adsorbent remains wet throughout the chromatographic separation process. This is crucial for a couple of reasons:
1. Maintaining an even flow of the elution solvent: The elution solvent is responsible for carrying the sample components through the column. If the level of the elution solvent drops below the top of the adsorbent, air may be introduced into the column. This can disrupt the flow of the solvent and create irregularities in the separation process. By keeping the adsorbent wet, a smooth and continuous flow of the solvent can be maintained, allowing for consistent and reliable separation.
2. Preventing sample degradation or loss: Some samples may be sensitive to air or prone to evaporation. If the elution solvent level drops below the top of the adsorbent, the sample components may be exposed to air or dry out. This can lead to degradation or loss of the sample, compromising the accuracy and reliability of the chromatographic analysis.
To ensure that the level of the elution solvent remains above the top of the adsorbent, it is necessary to monitor the solvent level during the chromatographic run and adjust it as needed by adding more solvent to the column reservoir. This helps maintain optimal chromatographic conditions and ensures successful separation of the sample components.