5’-CGCACCTGTGTTGATCACCTAGCCGATCCACGGTGGATCCAAGGC-3’

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3’-GCGTGGACACAACTAGTGGATCGGCTAGGTGCCACCTAGGTTCCG-5’

Tip: If you want to see a review of PCR, we recommend this amazing animation and virtual lab made by the University of Utah.

You also have a collection of short DNA primers:

Primer 1: 5’-CGTGGA-3’
Primer 2: 5’-TGTGTT-3’
Primer 3: 5’-ATCCAA-3’
Primer 4: 5’-CCTTGG-3’
You put together several reaction mixtures, run them in the thermocycler under conditions that should produce a product if the reaction mixture is correct.

All mixtures contain:

5’-CGCACCTGTGTTGATCACCTAGCCGATCCACGGTG
GATCCAAGGC-3’

The 45 bp DNA fragment
DNA polymerase that can function at high temperature
The necessary buffer conditions
For each mixture, indicate whether or not the PCR reaction would produce a product. If a product would be produced, give the length of that product in base-pairs (bp); if no product would be produced, give zero “0” for the length.

1
Added: dATP, dGTP, dCTP, Primer 2, Primer 4
- unanswered

2
Added: dATP, dGTP, dCTP, dTTP, Primer 2
- unanswered
3
Added: dATP, dGTP, dCTP, dTTP, Primer 2, Primer 4
- unanswered
4
Added: dATP, dGTP, dCTP, dTTP, Primer 1, Primer 3
- unanswered
5
Added: dATP, dGTP, dCTP, dTTP, Primer 2, Primer 3

To determine whether or not a PCR reaction would produce a product for each mixture, we need to consider the primers and the presence of the specific DNA sequence in the reaction. Here's how we can analyze each mixture:

Mixture 1: Added dATP, dGTP, dCTP, Primer 2, Primer 4
- Primer 2 (5'-TGTGTT-3') matches with the 5'-TGTTGA-3' region of the DNA template.
- Primer 4 (5'-CCTTGG-3') matches with the 5'-CCTTGG-3' region of the DNA template.
- Both primers have binding sites in the DNA template, so a product would be produced.
- The product length would be the distance between the binding sites of the two primers, which is 6 base pairs (bp).

Mixture 2: Added dATP, dGTP, dCTP, dTTP, Primer 2
- Primer 2 (5'-TGTGTT-3') matches with the 5'-TGTTGA-3' region of the DNA template.
- There are no other primers added, so there is no second primer binding site in the DNA template.
- Without a second primer binding site, no product would be produced. The length would be 0 bp.

Mixture 3: Added dATP, dGTP, dCTP, dTTP, Primer 2, Primer 4
- Primer 2 (5'-TGTGTT-3') matches with the 5'-TGTTGA-3' region of the DNA template.
- Primer 4 (5'-CCTTGG-3') matches with the 5'-CCTTGG-3' region of the DNA template.
- Both primers have binding sites in the DNA template, so a product would be produced.
- The product length would be the distance between the binding sites of the two primers, which is 6 bp.

Mixture 4: Added dATP, dGTP, dCTP, dTTP, Primer 1, Primer 3
- Primer 1 (5'-CGTGGA-3') does not have a perfect match in the DNA template.
- Primer 3 (5'-ATCCAA-3') does not have a perfect match in the DNA template.
- Neither primer has a binding site in the DNA template, so no product would be produced. The length would be 0 bp.

Mixture 5: Added dATP, dGTP, dCTP, dTTP, Primer 2, Primer 3
- Primer 2 (5'-TGTGTT-3') matches with the 5'-TGTTGA-3' region of the DNA template.
- Primer 3 (5'-ATCCAA-3') does not have a perfect match in the DNA template.
- Only one primer has a binding site in the DNA template, so no product would be produced. The length would be 0 bp.

Therefore, the results for each mixture are as follows:

1. Product length: 6 bp
2. Product length: 0 bp
3. Product length: 6 bp
4. Product length: 0 bp
5. Product length: 0 bp