Posted by pys on .
Consider the following 45 base-pair (bp) DNA sequence:
1 10 20 30 40
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Tip: If you want to see a review of PCR, we recommend this amazing animation and virtual lab made by the University of Utah.
You also have a collection of short DNA primers:
Primer 1: 5’-CGTGGA-3’
Primer 2: 5’-TGTGTT-3’
Primer 3: 5’-ATCCAA-3’
Primer 4: 5’-CCTTGG-3’
You put together several reaction mixtures, run them in the thermocycler under conditions that should produce a product if the reaction mixture is correct.
All mixtures contain:
The 45 bp DNA fragment
DNA polymerase that can function at high temperature
The necessary buffer conditions
For each mixture, indicate whether or not the PCR reaction would produce a product. If a product would be produced, give the length of that product in base-pairs (bp); if no product would be produced, give zero “0” for the length.
Reaction Added to Mixture Length of Resulting Product
dATP, dGTP, dCTP, dTTP