Differences between bacterial and mammalian transcription?
Since this is not my area of expertise, I searched Google under the key words " bacterial vs. mammalian transcription" to get these possible sources:
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In the future, you can find the information you desire more quickly, if you use appropriate key words to do your own search. Also see http://hanlib.sou.edu/searchtools/.
Bacterial and mammalian transcription are two different processes that occur in different types of organisms, involving distinct molecular machinery and mechanisms. Here are the key differences between bacterial and mammalian transcription:
1. Complexity: Bacterial transcription is relatively simpler compared to mammalian transcription. Bacteria have a single RNA polymerase enzyme responsible for synthesizing all types of RNA molecules. In contrast, mammals have multiple types of RNA polymerases, each specialized for different classes of RNA molecules.
2. Promoter recognition: Bacterial transcription initiation is primarily regulated by a sigma factor protein that binds to a specific DNA sequence called the promoter. In mammals, transcription initiation is more complex and involves interactions between a variety of proteins, known as transcription factors, that bind to specific DNA sequences known as enhancers and promoters.
3. Transcription termination: In bacteria, transcription usually terminates at specific sequences within the DNA called terminators, which cause the RNA polymerase to detach from the DNA template. In mammals, transcription termination is more diverse and can involve different mechanisms, such as cleavage and polyadenylation signals.
4. Regulation: Bacterial transcription regulation often involves negative control mechanisms, where regulatory proteins called repressors bind to the DNA and prevent transcription. Mammalian transcription regulation is more complex and can involve both negative control (repression) and positive control (activation), where regulatory proteins bind to specific DNA sequences and influence the activity of RNA polymerase.
To investigate these differences further, researchers often perform experiments such as biochemical assays, genetic manipulations, and comparative genomics.