ou sequence many of the recombinant plasmids generated. You find three mutant versions of the inserted gene. (This is likely due to errors introduced during the PCR amplification.) The sequences of the gene found in the three mutants are given below.
Consider each of the following mutations separately.
i) Mutant A: Suppose that the T/A base pair at position 27 and indicated by a line was changed to a G/C base pair. What would the amino acid sequence of the resulting protein be?
Be sure to enter the sequence N- to -C from left to right. Do not include the "N-" or "-C". Please use the 3-letter amino acid abbreviations (for example, "Ala-Gly-Ser"). We will ignore spaces and dashes when we grade your answer.
To determine the amino acid sequence of the resulting protein after the mutation in Mutant A, we need to first analyze the change in the DNA sequence and then translate it into an amino acid sequence.
Given that the T/A base pair at position 27 in the original sequence has been changed to a G/C base pair, we can represent the mutation as follows:
Original sequence: ...T/A... (position 27)
Mutant sequence: ...G/C... (position 27)
To determine the effect of this mutation on the amino acid sequence, we need to refer to the genetic code. The genetic code is a set of rules that determines how codons (three nucleotide sequences) are translated into specific amino acids.
In this case, we need to look at the codon that corresponds to the mutated position 27. The codons are read in a 3-letter order (e.g., ATG).
The codon chart shows that the codon TAC codes for the amino acid Tyrosine (Tyr) and the codon GGC codes for the amino acid Glycine (Gly).
So, the resulting amino acid sequence of the protein in Mutant A, considering the mutation, would be:
Original sequence: ...Tyr...
Mutant sequence: ...Gly...
Therefore, the resulting amino acid sequence of the protein in Mutant A, considering the T/A to G/C mutation at position 27, would be "Tyr-Gly".