) Anthranilate fluoresces blue under ultraviolet light. Under normal conditions, the levels of anthranilate in bacterial cells are low and the cells do not visibly fluoresce. However, if anthranilate accumulates because a relevant enzyme is defective or absent, the cells will fluoresce.
The table below lists several enzymes in the tryptophan biosynthetic pathway. Each row corresponds to a different bacterial strain. A “+” in the table indicates that the gene for that enzyme is functional in that strain. A “-“ in the table indicates that the gene for that enzyme is non-functional in that strain. For each strain, indicate whether or not you would expect to see blue fluorescence under ultraviolet light.
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If you are taking Lander's testin edx, the answers are no no yes no yes
To determine whether or not you would expect to see blue fluorescence under ultraviolet light in each strain, you need to examine the presence or absence of the enzymes in the tryptophan biosynthetic pathway.
The tryptophan biosynthetic pathway is a series of enzymes responsible for the synthesis of tryptophan, an amino acid. Anthranilate is an intermediate compound in this pathway.
Here is a breakdown of the enzymes in the pathway and their corresponding genes:
1. Enzyme 1 (+/-) - This refers to the presence or absence of the gene for Enzyme 1 in each strain.
2. Enzyme 2 (+/-) - This refers to the presence or absence of the gene for Enzyme 2 in each strain.
3. Enzyme 3 (+/-) - This refers to the presence or absence of the gene for Enzyme 3 in each strain.
4. Enzyme 4 (+/-) - This refers to the presence or absence of the gene for Enzyme 4 in each strain.
5. Enzyme 5 (+/-) - This refers to the presence or absence of the gene for Enzyme 5 in each strain.
Based on the information provided in the table, you can determine whether blue fluorescence would be expected in each strain by looking at the status of the genes for these enzymes.
If the gene for any of the enzymes in the pathway is non-functional (-), it means that the enzyme is defective or absent, and the corresponding step in the tryptophan biosynthetic pathway would not occur. Without that step, anthranilate would not be synthesized, leading to low levels of anthranilate in bacterial cells. Consequently, there would be no visible blue fluorescence under ultraviolet light.
On the other hand, if all the genes for the enzymes in the tryptophan biosynthetic pathway are functional (+), it means that each step in the pathway is occurring correctly, leading to the synthesis of tryptophan and anthranilate. In this case, if anthranilate accumulates due to a defect or absence of a relevant enzyme, the cells would visibly fluoresce blue under ultraviolet light.
By examining the presence or absence of the genes for the enzymes in the tryptophan biosynthetic pathway for each strain in the table, you can determine whether blue fluorescence would be expected or not for each strain.