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May 22, 2015

Homework Help: Biochemistry

Posted by OR on Monday, October 7, 2013 at 5:29pm.

Can someone help me calculate protein concentration using the Bradford Assay?

If you could show me how to do tube 2, that'd be great.

Tube 1: 0 ul Protein Stock Solution, 800 ul Water, 0 ug Protein, Absorbance @595nm = 0
Tube 2: 10 ul Protein Stock Solution, 790 ul Water, 1.4 ug Protein, Absorbance @595nm = .068
Tube 3: 20 ul Protein Stock Solution, 780 ul Water, 2.8 ug Protein, Absorbance @595nm = .144
Tube 4: 40 ul Protein Stock Solution, 760 ul Water, 5.6 ug Protein, Absorbance @595nm = .256
Tube 5: 60 ul Protein Stock Solution, 740 ul Water, 8.4 ug Protein, Absorbance @595nm = .361
Tube 6: 80 ul Protein Stock Solution, 720 ul Water, 11.2 ug Protein, Absorbance @595nm = .464

I have to determine the protein concentration and plot it against the absorbance.

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