Thursday
August 21, 2014

Homework Help: Biochemistry

Posted by OR on Monday, October 7, 2013 at 5:29pm.

Can someone help me calculate protein concentration using the Bradford Assay?

If you could show me how to do tube 2, that'd be great.

Tube 1: 0 ul Protein Stock Solution, 800 ul Water, 0 ug Protein, Absorbance @595nm = 0
Tube 2: 10 ul Protein Stock Solution, 790 ul Water, 1.4 ug Protein, Absorbance @595nm = .068
Tube 3: 20 ul Protein Stock Solution, 780 ul Water, 2.8 ug Protein, Absorbance @595nm = .144
Tube 4: 40 ul Protein Stock Solution, 760 ul Water, 5.6 ug Protein, Absorbance @595nm = .256
Tube 5: 60 ul Protein Stock Solution, 740 ul Water, 8.4 ug Protein, Absorbance @595nm = .361
Tube 6: 80 ul Protein Stock Solution, 720 ul Water, 11.2 ug Protein, Absorbance @595nm = .464

I have to determine the protein concentration and plot it against the absorbance.

Answer this Question

First Name:
School Subject:
Answer:

Related Questions

Biochemistry - A protein sample is diluted 5-fold before measuring the protein ...
Physical Chemistry - A 100 amino acid folded protein (F) is in equilibrium with ...
chemistry - Expressing concentration in units of μg/mL or mg/mL are common ...
Chemistry - 1)Buttermilk has 9 grams of protein and 12 grams of carbohydrate per...
Biochem - If the calibration curve for a Beer's Law plot of a protein assay had...
Chem - If the calibration curve for a Beer's Law plot of a protein assay had a ...
Biology Lab - Can you please show me how to answer this question using C1V1=C2V2...
chemistry - Buttermilk has 9 grams of protein and 12 grams of carbohydrate per ...
Biochemistry - Have two quick questions... Why does denatured protein ...
Biochemistry - On preparation of a solution of protein, you attempted to ...

Search
Members