11. You need to make a 1:8 ratio of DNA of two DNA types A and B (A:B). Your total aliquot should be 2ng/26 ìL. For each of the following sets, calculate how much of each extract you should use and how much TE-4:

a. Extract A: 2.5 ng/20 ìL, Extract B: 252.3 pg/ìL

b. Extract A: 36 pg/ìL, Extract B: 533.0 pg/ìL (1/100 dilution)

To calculate how much of each DNA extract and TE-4 you should use to achieve the desired 1:8 ratio, follow these steps:

Step 1: Calculate the total amount of DNA in the final aliquot:
For a 1:8 ratio, the total amount of DNA in the final aliquot should be 2ng/26 ìL.

Step 2: Calculate the amount of DNA extract needed for each type:
To determine the amount of each DNA extract to use, multiply the total amount of DNA in the final aliquot by the corresponding ratio proportion.
Ratio A:B = 1:8
Let's assume the amount of extract A to be X and the amount of extract B to be Y.

Amount of Extract A needed = X
Amount of Extract B needed = 8Y

We can set up the following equation:
2ng/26 ìL = X + 8Y

Step 3: Calculate the amount of TE-4 needed to make up the total aliquot volume:
The remaining volume in the final aliquot will be filled with TE-4. Therefore, the TE-4 volume will be the total aliquot volume minus the total volume of DNA extracts.

TE-4 volume = Total aliquot volume - Volume of Extract A - Volume of Extract B

For this question, the total aliquot volume is 26 ìL.

a. For the first set:
Extract A: 2.5 ng/20 ìL, Extract B: 252.3 pg/ìL

Step 1:
Total DNA needed = 2ng/26 ìL

Step 2:
Convert the DNA amount in Extract A to ng:
Extract A: 2.5 ng/20 ìL = (2.5/20) ng/ìL

X = (2.5/20) ng/ìL
Y = 8 * (252.3/1000) ng/ìL (Converting pg to ng by dividing by 1000)

Step 3:
TE-4 volume = 26 ìL - Volume of Extract A - Volume of Extract B

b. For the second set:
Extract A: 36 pg/ìL, Extract B: 533.0 pg/ìL (1/100 dilution)

Step 1:
Total DNA needed = 2ng/26 ìL

Step 2:
Convert the DNA amount in Extract A to ng:
Extract A: 36 pg/ìL = (36/1000) ng/ìL (Converting pg to ng by dividing by 1000)

X = (36/1000) ng/ìL
Y = 8 * (533.0/1000) ng/ìL (Converting pg to ng by dividing by 1000)

Step 3:
TE-4 volume = 26 ìL - Volume of Extract A - Volume of Extract B

Please calculate the volumes accordingly using the values obtained in steps 2 and 3.