You plan to generate many copies of this gene by PCR. You will then insert the PCR product into the plasmid.
d) Which two primers from the list below will allow you to use PCR to amplify this gene? Check the two that apply.
5'-GTGCA-3'
5'-CACGT-3'
5'-TATGG-3'
5'-CCATA-3'
5'-ATACC-3'
5'-GGTAT-3'
To determine which primers will allow you to amplify this gene by PCR, you need to carefully examine the target gene sequence and determine the complementary regions that can be used as binding sites for the primers.
Looking at the given primer sequences:
- 5'-GTGCA-3'
- 5'-CACGT-3'
- 5'-TATGG-3'
- 5'-CCATA-3'
- 5'-ATACC-3'
- 5'-GGTAT-3'
You need to compare these primers with the target gene sequence to see if they have complementary regions. However, you haven't provided the target gene sequence, so I am unable to determine which primers are suitable for amplification.
To find out which primers to use, you should have the target gene sequence available. Once you have the sequence, you can align each primer sequence with the target gene sequence to see if they match or have complementary regions. Ideally, the forward primer should bind to the 5' end of the target gene sequence, and the reverse primer should bind to the 3' end, allowing amplification of the gene in-between.
Without the target gene sequence, it is not possible to identify the specific primers that will amplify the gene. Please provide the target gene sequence, and I will assist you further in determining the appropriate primers for gene amplification through PCR.