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Alcanivorax is a naturally occurring oil-degrading marine bacterium that can quickly become the predominant microbe in oil-contaminated open oceans and coastal waters. To identify the genes important for the oil-degrading ability, you could attempt the following:
1) You might begin by identifying mutant Alcanivorax cells with a specific phenotype. For what mutant phenotype would you screen? Answer by completing the following statement: The mutant cells would not be able to...
2) For your second step you might make a bacterial DNA library. What type of bacterial cells would you use as a source for the genomic DNA library?
3) Finally, you could transform the mutant cells identified with the bacterial DNA library as given above. How would you identify cells that have a recombinant plasmid carrying a gene involved in oil degradation?